Publications

原著論文

• Matsuhashi, K., Ito, K., Nagai, K., Sanada, A., Watanabe, K., Takumi, K., Toyoda, A., Fukuyama, M., Yamamoto, S., Chinen, T., Stewart, G., Hata, S.* and Kitagawa, D.* (2025)
• The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication
• Nature Communications
• DOI: 10.1038/s41467-025-63002-3 (*corresponding authors)
  Press release: https://www.u-tokyo.ac.jp/focus/ja/press/z0111_00089.html

• #Tei, C., #Hata, S.*, #Mabuchi, A., Okuda, S., Ito, K., Genova, M., Fukuyama, M., Yamamoto, S., Chinen, T., Toyoda, A. and Kitagawa, D.* (2025)
• Comparative analysis of multiple DNA double-strand break repair pathways in CRISPR-mediated endogenous tagging
• Communications Biology
• DOI: 10.1038/s42003-025-08187-5 (*corresponding authors, #co-first authors)

• Ito, K., Takumi, K., Matsuhashi, K., Sakamoto, H., Nagai, K., Fukuyama, M., Yamamoto, S., Chinen, T., Hata, S.* and Kitagawa, D.* (2025)
• Multimodal mechanisms of human centriole engagement and disengagement
• The EMBO Journal
• DOI: 10.1038/s44318-024-00350-8 (*corresponding authors)
  Press release: https://www.u-tokyo.ac.jp/focus/ja/press/z0111_00076.html

• Takeda, Y., Chinen, T.*, Honda, S., Takatori, S., Okuda, S., Yamamoto, S., Fukuyama, M., Takeuchi, K., Tomita, T., Hata, S.* and Kitagawa, D.* (2024)
• Molecular basis promoting centriole triplet microtubule assembly
• Nature Communications
• DOI: 10.1038/s41467-024-46454-x (*corresponding authors)
  Press release: https://www.u-tokyo.ac.jp/focus/ja/press/z0111_90050.html

• Ito, K.*, Tsuruoka, Y.* and Kitagawa, D.* (2024)
• Predicting potential target genes in molecular biology experiments using machine learning and multifaceted data sources
• iScience
• DOI: 10.1016/j.isci.2024.109309 (*corresponding authors)

• #Harada, T., #Hata, S.*, Takagi, R., Komori, T., Fukuyama, M., Chinen, T. and Kitagawa, D.* (2023)
• An antioxidant screen identifies ascorbic acid for prevention of light-induced mitotic prolongation in live cell imaging
• Communications Biology
• DOI: 10.1038/s42003-023-05479-6 (*corresponding authors, #co-first authors)

• #Mabuchi, A., #Hata, S.*, Genova, M., Tei, C., Ito, K., Hirota, M., Komori, T., Fukuyama, M., Chinen, T., Toyoda, A. and Kitagawa, D.* (2023)
• ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells
• BMC Genomics
• DOI: 10.1186/s12864-023-09377-3 (*corresponding authors, #co-first authors)

• #Komori, T., #Hata, S.*, Mabuchi, A., Genova, M., Harada, T., Fukuyama, M., Chinen, T. and Kitagawa, D.* (2023)
• A CRISPR-del-based pipeline for complete gene knockout in human diploid cells
• Journal of Cell Science
• DOI: 10.1242/jcs.260000 (*corresponding authors, #co-first authors)

• Tanaka, A., Nakano, T., Watanabe, K., Masuda, K., Honda, G., Kamata, S., Yasui, R., Kozuka-Hata, H., Watanabe, C., Chinen, T., Kitagawa, D., Sawai, S., Oyama, M., Yanagisawa M. and Kunieda, T.* (2022)
• Stress-dependent cell stiffening by tardigrade tolerance proteins that reversibly form a filamentous network and gel
• PLOS Biology
• DOI: 10.1371/journal.pbio.3001780 (*corresponding authors)

• Yamamoto, S., Yabuki, R. and Kitagawa, D.* (2021)
• Biophysical and biochemical properties of Deup1 self-assemblies: a potential driver for deuterosome formation during multiciliogenesis
• Biology Open
• DOI: 10.1242/bio.056432 (*corresponding authors)

• #Ito, K., #Watanabe, K., Ishida, H., Matsuhashi, K., Chinen, T., Hata, S. and Kitagawa, D.* (2021)
• Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle
• Journal of Cell Biology
• DOI: 10.1083/jcb.202005153 (*corresponding authors, #co-first authors)

• #Chinen, T.*, #Yamazaki, K., Hashimoto, K., Fujii, K., Watanabe, K., Takeda, Y., Yamamoto, S., Nozaki, Y., Tsuchiya, Y., Takao, D. and Kitagawa, D.* (2021)
• Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly
• Journal of Cell Biology
• DOI: 10.1083/jcb.202006085 (*corresponding authors, #co-first authors)

• Takeda, Y., Yamazaki, K. Hashimoto, K. Watanabe, K. Chinen, T.* and Kitagawa, D.* (2020)
• The centriole protein CEP76 negatively regulates PLK1 activity in the cytoplasm for proper mitotic progression
• Journal of Cell Science
• DOI: 10.1242/jcs.241281 (*corresponding authors)

• Chinen, T., Yamamoto, S., Takeda, Y., Watanabe, K., Kuroki, K., Hashimoto, K., Takao, D. and Kitagawa, D.* (2020)
• NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells.
• The EMBO Journal
• DOI: 10.15252/embj.2019102378 (*corresponding authors)

• Takao, D.*, Watanabe, K., Kuroki, K. and Kitagawa, D.* (2019)
• Feedback loops in the Plk4–STIL–HsSAS6 network coordinate site selection for procentriole formation.
• Biology Open
• DOI: 10.1242/bio.047175 (*corresponding authors)

• Takao, D.*, Yamamoto, S. and Kitagawa, D.* (2019)
• A Theory of Centriole Duplication Based on Self-Organized Spatial Pattern Formation.
• Journal of cell biology
• DOI: 10.1083/jcb.201904156 (*corresponding authors)

• Yoshiba, S., Tsuchiya, Y., Ohta, M., Gupta, A., Shiratsuchi, G., Nozaki, Y., Ashikawa, T., Fujiwara, T., Natsume, T., Kanemaki, M. and Kitagawa, D.* (2019)
• HsSAS-6-dependent cartwheel assembly ensures stabilization of centriole intermediates.
• Journal of cell science
• DOI: 10.1242/jcs.217521 (*corresponding authors)

• Yamamoto, S. and Kitagawa, D.* (2019)
• Self-organization of Plk4 regulates symmetry breaking in centriole duplication.
• Nature Communications
• DOI: 10.1038/s41467-019-09847-x (*corresponding authors)
• 詳細はこちら(日本語要旨)

• Watanabe, K., Takao, D., Ito, K., Takahashi, M. and Kitagawa, D.* (2019)
• The Cep57-pericentrin module organizes PCM expansion and centriole engagement.
• Nature Communications
• DOI: 10.1038/s41467-019-08862-2 (*corresponding authors)
• 詳細はこちら(日本語要旨)

• Ohta, M., Watanabe, K., Ashikawa, T., Nozaki, Y., Yoshiba, S., Kimura, A. and Kitagawa, D.* (2018)
• Bimodal Binding of STIL to Plk4 Controls Proper Centriole Copy Number.
• Cell Reports
• DOI: 10.1016/j.celrep.2018.05.030 (*corresponding authors)

• #Okamoto, N.*, #Tsuchiya, Y., Miya, F., Tsunoda, T., Yamashita, K., Boroevich, KA., Kato, M., Saitoh, S., Yamasaki, M., Kanemura, Y., Kosaki, K. and Kitagawa, D.* (2017)
• A novel genetic syndrome with STARD9 mutation and abnormal spindle morphology.
• American Journal of Medical Genetics Part A
• DOI: 10.1002/ajmg.a.38391 (*corresponding authors, #co-first authors)

• Gupta, A., Tsuchiya, Y., Ohta, M., Shiratsuchi, G. and Kitagawa, D.* (2017)
• NEK7 is required for G1 progression and procentriole formation.
• Molecular Biology of the Cell
• (*corresponding authors)

• #Okamoto, N.*, #Tsuchiya, Y., Kuki, I., Yamamoto, T., Saitsu, H., Kitagawa, D.* and Matsumoto, N.* (2017)
• Disturbed chromosome segregation and multipolar spindle formation in a patient with CHAMP1 mutation.
• Molecular Genetics and Genomic Medicine
• (*corresponding authors, #co-first authors)

• Jin, M., Pomp, O., Shinoda, T., Toba, S., Torisawa, T., Furuta, K., Oiwa, K., Yasunaga, T., Kitagawa, D., Matsumura, S., Miyata, T., Tan, T.T., Reversade, B.* and Hirotsune, S.* (2017)
• Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics.
• Scientific Reports
• DOI: 10.1038/srep39902 (*corresponding authors)

• Tsuchiya, Y., Yoshiba, S., Gupta, A., Watanabe, K. and Kitagawa, D.* (2016)
• Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole.
• Nature Communications
• DOI: 10.1038/ncomms12567 (*corresponding authors)

• Matsuura, R., Ashikawa, T., Nozaki, Y. and Kitagawa, D.* (2016)
• LIN-41 inactivation leads to delayed centrosome elimination and abnormal chromosome behavior during female meiosis in Caenorhabditis elegans.
• Molecular Biology of the Cell
• (*corresponding authors)

• Zitouni, S.*, Francia, M.E.*, Leal, F., Montenegro Gouveia, S., Nabais, C., Duarte, P., Gilberto, S., Brito, D., Moyer, T., Kandels-Lewis, S., Ohta, M., Kitagawa, D., Holland, A.J., Karsenti, E., Lorca, T., Lince-Faria, M. and Bettencourt-Dias, M.* (2016)
• CDK1 prevents unscheduled PLK4-STIL complex assembly in centriole biogenesis.
• Current Biology
• (*corresponding authors)

• Shiratsuchi, G., Takaoka, K., Ashikawa, T., Hamada, H. and Kitagawa, D.* (2015)
• RBM14 prevents assembly of centriolar protein complexes and maintains spindle integrity.
• EMBO Journal
• (*corresponding authors)

• Shiratsuchi, G. and Kitagawa, D.* (2015)
• Suppression of the ectopic assembly of centriole proteins ensures mitotic spindle integrity.
• Molecular and Cellular Oncology
• DOI: 10.1080/23723556.2014.1002717 (*corresponding authors)

• Ohta, M., Ashikawa, T., Nozaki, Y., Kozuka-Hata, H., Goto, H., Inagaki, M., Oyama, M. and Kitagawa, D.* (2014)
• Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole.
• Nature Communications
• DOI: 10.1038/ncomms6267 (Open access) (*corresponding authors)

• Kitagawa, D., Kohlmaier, G., Keller, D., Strnad, P., Balestra, F.R., Flückiger, I. and Gönczy, P.* (2011)
• Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL.
• Journal of Cell Science
• (*corresponding authors)

• Kitagawa, D., Vakonakis, I., Olieric, N., Hilbert, M., Keller, D., Olieric, V., Bortfeld, M., Erat, M.C., Flückiger, I., Gönczy, P.* and Steinmetz, M.O. (2011)
• Structural basis of the 9-fold symmetry of centrioles.
• Cell
• DOI: 10.1016/j.cell.2011.01.008 (*corresponding authors)

• Kitagawa, D., Flückiger, I., Polanowska, J., Keller, D., Reboul, J. and Gönczy, P.* (2011)
• PP2A phosphatase acts upon SAS-5 to ensure centriole formation in C. elegans embryos.
• Developmental cell
• (*corresponding authors)

• Kitagawa, D., Busso, C., Flückiger, I. and Gönczy, P.* (2009)
• Phosphorylation of SAS-6 by ZYG-1 is critical for centriole formation in C. elegans embryos.
• Developmental cell
• (*corresponding authors)

• (You can find other papers in Pubmed)

総説・その他

• #Ryosuke, A., #Mabuchi, A. and Hata, S.* (2025)
• Coiled-coils as emerging drivers of liquid–liquid phase separation
• The Journal of Biochemistry
• DOI: 10.1093/jb/mvaf065 (*corresponding authors, #co-first authors)

• #Miyazawa, M., #Kawai, K., #Yamamoto, S.* and Kitagawa, D. (2025)
• Protocol for immunostaining of non-adherent cells and cellular structures using centrifugal filter devices
• STAR Protocols
• DOI: 10.1016/j.xpro.2025.103934 (*corresponding authors, #co-first authors)

• Okuda, S., Chinen, T.* and Kitagawa, D. (2025)
• A Method for Analyzing Acentrosomal Mitotic Spindles in Human Cells.
• Methods in Molecular Biology
• DOI: 10.1007/978-1-0716-4224-5_15 (*corresponding authors)

• Miyazawa, M., Yamamoto, S.*, Goyama, S. and Kitagawa, D. (2025)
• Isolation of Mitotic Centrosomes from Cultured Human Cells.
• Methods in Molecular Biology
• DOI: 10.1007/978-1-0716-4224-5_16 (*corresponding authors)

• 伊藤慶, 鶴岡慶雅, 北川大樹 (2022)
• 任意のヒト遺伝子に関わる生命科学実験の検索・提案ウェブツール「LEXAS」
• 実験医学, vol. 41, No.1, 102-108

• 新倉竜太, 知念拓実 (2022)
• CRISPRスクリーニングデータベースを活用した創薬標的探索
• 生物工学会誌, 第100巻, 第8号, 449. 2022
• DOI: 10.34565/seibutsukogaku.100.8_449

• 竹田穣, 知念拓実, 北川大樹 (2022)
• 中心体タンパク質による分裂期PLK1制御を介した適切な細胞分裂保証メカニズム
• 生化学, 6月号掲載, vol.94, No.3 2022, 419-422

• Takumi, K. and Kitagawa, D. (2022)
• Experimental and Natural Induction of de novo Centriole Formation
• Frontiers in Cell and Developmental Biology
• DOI: 10.3389/fcell.2022.861864

• 北川大樹 (2022)
• 中心体
• 遺伝学の百科事典・継承と多様性の源, 日本遺伝学会編

• 馬渕陽, 畠星治, 北川大樹 (2021)
• 相分離を介した中心体の制御機構
• 実験医学増刊, vol.39, No.10, 110-115

• Yamamoto, S. and Kitagawa, D. (2020)
• Emerging insights into symmetry breaking in centriole duplication: updated view on centriole duplication theory
• Current Opinion in Structural Biology
• DOI: 10.1016/j.sbi.2020.08.005

• 松橋恭平, 北川大樹 (2020)
• 中心体の構造と細胞分裂
• 生体の科学, 2020 7月号掲載, vol. 71 No.4 338-342

• Takeda, Y., Kuroki, K., Chinen, T. and Kitagawa, D. (2020)
• Centrosomal and Non-centrosomal Functions Emerged through Eliminating Centrosomes
• Cell Structure and Function
• DOI: 10.1247/csf.20007

• Hashimoto, K., Chinen, T. and Kitagawa, D. (2020)
• Mechanisms of spindle bipolarity establishment in acentrosomal human cells
• Molecular & Cellular Oncology
• DOI: 10.1080/23723556.2020.1743899

• Ito, K., Watanabe, K., and Kitagawa, D. (2020)
• The Emerging Role of ncRNAs and RNA-Binding Proteins in Mitotic Apparatus Formation
• Non-Coding RNA
• DOI: 10.3390/ncrna6010013

• 竹田穣, 知念拓実, 北川大樹 (2019)
• 分裂期紡錘体形成を阻害する抗がん薬開発の動向
• ファルマシア, 10月号掲載, Vol.55 No.10 2019. 954-958

• Gupta, A. and Kitagawa, D. (2018)
• Ultrastructural diversity between centrioles of eukaryotes.
• Journal of Biochemistry
• DOI: 10.1093/jb/mvy031

• 北川大樹 (2017)
• 進化的に保存された中心体の複製と成熟過程の分子機構
• 生化学, ８月号掲載, vol. 89, No.4, 489-497

• 北川大樹 (2013)
• 中心小体構築と複製の分子メカニズム
• 細胞工学, vol. 32, No.3, 285-290

• 北川大樹 (2012)
• 中心小体複製の分子機構
• 生化学, 2月号掲載

• 北川大樹 (2011)
• 中心小体再構成のスナップショット
• 細胞工学, vol. 30, No.11, 1127

• 北川大樹, Steinmetz, M. O. and Gönczy, P. (2011)
• 中心小体複製開始の分子メカニズム
• 実験医学, vol. 29, No.11, 1777-1780

• 北川大樹, Steinmetz, M. O., and Gönczy, P. (2011)
• 進化上保存された中心小体の９回対称構造の謎を解く
• 細胞工学, vol. 30, No.5, 534-5